Transfection Reagents
Lonza Walkersville Mouse Neural Stem Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary mouse neural stem cells (NSCs).The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Neural Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit L
The SE Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System (including 4D-Nucleofector including LV Unit), 96-well Shuttle System and 384-well Nucleofector System Applications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL SE Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel,24 x Single use pipettes
DIASYS DIAGNOSTIC SYSTEMS USA LLC DiaSys ASAT FS is a ready-to-use two component photometric reagent to determine Aspartate Aminotransferase in serum or heparin plasma on photometric systems. Each set contains R1=10L, R2=2.5L
Diagnostic reagent for quantitative in vitro determination of Aspartate Aminotransferase (ASAT, AST) in animal serum or heparin plasma on photometric systems. For veterinary or research use only. Increased ASAT, formerly called Glutamic Pyruvic Transaminase (GPT), levels may occur in connection with damages of heart or skeletal muscle as well as of liver parenchyma. Parallel measurement of ALAT and ASAT is recommended to distinguish liver from heart or skeletal muscle damages. Method: Optimized UV-test according to IFCC (International Federation of Clinical Chemistry and Laboratory Medicine). The reagent has a wide measuring range from 1.2 U/L to 600 U/L and very good precision. Reagent 1 contains TRIS, L-Aspartate Malate Dehydrogenase and Lactate Dehydrogenase, reagent 2 contains 2-Oxogluterate and NADH. Reagents must be stored refrigerated.
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE 9 DNA Transfection Reagent, 0.4 mL
X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3958052 CACO-2 CELL LINE HUMAN
Boca Scientific Inc ViroMag R/L Viral Gene Delivery Reagent for Retrovirus/Lentivirus, 1ml
ViroMag R/L Transduction Reagent is a magnetic nanoparticles formulation optimized for Retroviruses and Lentiviruses. Based on Magnetofection technology, this reagent allows concentration of the complete applied dose of Retro/Lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag R/L is ideal for infecting non-permissive cells. It demonstrates an exceptionally high efficiency in promoting, controlling and assisting viral transductions. No molecular biology processes or biochemical modifications are required. This reagent must be used with a magnetic plate.
Genesee Scientific Corporation jetPEI HTS DNA Transfection Reagent, Low Toxicity, High Throughput Screening Compatible, 5 °C ± 3°C
jetPEI HTS DNA Transfection Reagent is a linear polyethylenimine derivative designed for efficient gene delivery in High Throughput Screening (HTS) applications. It features low toxicity, and compatibility with serum and antibiotics, enhancing experimental flexibility. Its exceptional reproducibility ensures reliable results across experiments, making it an ideal choice for both automated and manual workflows.
Sigma Aldrich Fine Chemicals Biosciences DOTAP Liposomal Transfection Reagent | 144189-73-1 | MFCD16660441 | 5 x 400 uL
DOTAP Liposomal Transfection Reagent | Purity: 99% | Mol Wt: 774.19 | 144189-73-1 | MFCD16660441 | 5 x 400 uL
Expression Systems Transfection Medium, 100ml
Expression Systems Transfection Medium is designed to complement ESF 921 and ESF AF insect cell culture media for the cotransfection or transfection step in baculovirus vector production. Transfection Medium is a serum-free and animal-free formulation that facilitates vector DNA uptake by the insect host cells. Realize higher titers by using a formulation designed to work with ESF 921 and ESF AF.
• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Appropriate for PEI or lipofection mediated transfection
• Optimized for use with Expres2 TR Transfection Reagent
• Increases virus vector production and transient expression
• Available in 20 mL or 100 mL
• In stock for immediate shipment
• To download Instructions for Use and/or COA please visit: www.expressionsystems.com
Invivogen dsDNA Naked, Complexed with Transfection Reagent, 100 µg
Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt. It is a repetitive synthetic double-stranded DNA sequence of poly(dA-dT); poly(dT-dA) and a synthetic analog of B-DNA. Poly(dA:dT) is recognized by several sensors, including DAI, LRRFIP1 and AIM2. Poly(dA:dT) is also indirectly detected by RIG-I. The activity of poly(dA:dT) is tested using the reporter cell line B16-Blue IFN/. Solubility: 2 mg/ml in physiological water. Unit Size:100 g. 86828-69-5
VWR International IN VIVO-JETRNA+ TRANSFECTION R
in vivo-jetRNA+ transfection reagent, with mRNA buffer