Transfection Reagents
Apexbio Technology LLC Calcium Phosphate Cell Transfection Kit 200T
Calcium phosphate transfection is a commonly used method for introducing exogenous DNA into eukaryotic cells DNA-calcium phosphate precipitate adheres to cells by interacting with their cell surface The exogenous DNA is subsequently internalized via cellular endocytosis ultimately delivering the DNA into target cells This method has been successfully applied to various cell lines to achieve transient or stable transfections HEK293 cells are most suitable for calcium phosphate transfection Hela and CHO cells can also be transfected using the calcium phosphate method with slightly lower transfection efficiency This product significantly enhancing transfection efficiency and reducing cytotoxicity Additionally the kit provides highly consistent pH values and salt concentrations ensuring reliable transfection efficiency across different experiments and meet for the transfection needs of most adherent and suspension cells
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.
Active Motif Chariot™
Chariot is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
Cytek Biosciences Inc Cell Dispersal Reagent Amn
Product Name: Muse Cell Dispersal Reagent
Cytek Catalog Number: MCH100107
Product Class: Guava Muse Reagent
Type: Muse
Size: 100 Tests
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit S
The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SE Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0),2 x 16-well Nucleocuvette Strips (20 μL)
Kyfora Bio DMG-PEG2000 1 GR
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
Kyfora Bio DMG-PEG2000 100 MG
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.